UJI EFEK ANTIINFLAMASI EKSTRAK ETANOL DAUN PEPAYA (CARICA PEPAYA L.) PADA LUKA IRIS MENCIT JANTAN (MUS MUSCULUS)

Abstract

Abstract

Background: Wounds are disruptions in tissue continuity due to injury or surgery, which can affect the structure and function of tissues. According to RISKESDAS 2018, the prevalence of incised or cut wounds in Indonesia reached 20.1%. Wound healing can occur physiologically or with the help of medications. However, the use of synthetic anti-inflammatory drugs such as NSAIDs may cause side effects. Indonesia has great potential for utilizing medicinal plants as safer and more affordable alternative treatments, one of which is papaya leaf (Carica papaya L.), which contains flavonoids and papain enzymes with anti-inflammatory properties by inhibiting the cyclooxygenase enzyme in prostaglandin synthesis. Therefore, papaya leaf is expected to be developed as an alternative anti-inflammatory medicine.

Objective: This study aims to determine the anti-inflammatory effect of papaya leaf extract (Carica papaya L.) on the healing process of incised wounds in male mice (Mus musculus).

Methods: This research used a quantitative approach with a true experimental design through the posttest control group design method. The parameters observed were the average wound healing time (days) in each group of mice until the wound closed. The experimental animals were divided into several groups, namely a negative control that received no treatment, a positive control that was given 70% alcohol, and three treatment groups, namely the 15% papaya leaf ethanol extract group, the 20% papaya leaf ethanol extract group, and the 25% papaya leaf ethanol extract group.

Results: The evaluation of the anti-inflammatory effect of papaya leaf ethanol extract demonstrated that the 15% concentration group achieved the lowest mean wound healing score of 1.51 ± 1.51, representing the most optimal outcome compared to the other groups. This finding indicates that papaya leaf ethanol extract is effective in accelerating the healing process of incisional wounds in mice. Statistical analysis revealed a significant difference between the untreated control group and the 15% extract group (p < 0.05). In contrast, the other treatment groups did not show statistically significant differences, although a downward trend in wound scores was observed compared to the control groups..

Conclusion: The application of 15% papaya leaf ethanol extract was found to enhance the healing process of incisional wounds in mice and may serve as a promising natural anti-inflammatory agent.

Keywords: Papaya Leaf, Anti-inflammatory, Wound Healing, Inflammation, Incised Wound