Antihyperglycemic Effect of Combination of Ethanol Extract of Moringa Leaves (Moringa oleifera) and Chayote (Sechium edule (Jacq.) Swartz) on Male White Rats (Rattus norvegicus) Induced by Alloxan and HFD

Abstract

ABSTRACT
Background: Hyperglycemia is a marker for Diabetes Mellitus (DM) due to disorders of carbohydrate, fat and protein metabolism which are associated with insulin deficiency. The worsening morbidity and mortality from DM globally justifies the need for more diverse research into new therapies. The urgency of this research is very important, where a
model/formula of natural ingredients has been found that has pharmacological effects
which are expected to be able to overcome the mechanisms underlying Type 2 Diabetes Mellitus (T2DM). Another therapy for DM is consuming medicinal plants. Moringa leaves (Moringa oleifera) and Chayote (Sechium edule (Jacq.) Swartz) are medicinal plants that have antidiabetic properties. Objective: To analyze the antihyperglycemic effect of a combination of Moringa leaf
ethanol extract and Chayote ethanol extract on male white rats (Rattus norvegicus) induced by alloxan and High Fatty Diet (HFD). Research Methods: This research is laboratory experimental with a posttest only controlled group design on male white rats of the Wistar hyperglycemia strain which are divided into 6 groups, namely group K1 (normal), group K2 (negative control), group K3 (positive control), and therapy groups namely; group K4 (EEDK and EELS each 50 mg/KgBB), group K5 (EEDK 100 mg/KgBB + EELS 50 mg/KgBB), and group K6 (EEDK 50 mg/KgBB + EELS 100 mg/KgBB). Results: Reduction in body weight (BW), reduction in blood glucose levels (KGD), reduction in the percentage of blood glucose levels (%KGD), reduction in the Oral Glucose Tolerance Test (OGTT), and reduction in liver organ index were better in the K6 group (EEDK 50 mg /KgBW + EELS 100 mg/KgBW) compared to other treatment groups, except for the K3 group (positive control) with metformin. Reduction in Percentage Body Weight (% BW) and Lee index were better in the K4 group (EEDK and EELS 50 mg/KgBW each) compared to the other treatment groups, except in the K3 group (positive control) with metformin. Conclusion: The combination of EEDK and EELS in male white rats induced by alloxan and HFD had an antihyperglycemic effect and was able to reduce BW, KGD, % BW, % KGD, OGTT, and liver organ index. The dose of EEDK 50 mg/KgBW + EELS 100
mg/KgBW was more effective than groups K4 and K5 in reducing BW, KGD, % KGD, OGTT, and liver organ index. The EEDK and EELS doses of 50 mg/KgBW each were more effective than the K5 and K6 groups in reducing %BW and Lee index..